Seven thousand salmon fry, about four months-old and about one-and-a-half inches long, will be released into five small tributaries in the Lune catchment.
They were reared at a hatchery at Garsdale, near Sedbergh, from eggs taken from Lune salmon.
The fish will be added to the River Lune at Aikrigg Beck, Springs Gill, Black Horse Beck, Deep Gill Low Gill and Wasdale Beck.
At the same time the Environment Agency will also be putting about 4,400 sea trout fry into Aspland Beck – a tributary of the River Greta, near Ingleton. These fish are being stocked into the river to overturn the effects of a pollution incident that occurred last year.
John Cizdyn, Environment Agency Fisheries Officer, said: “The Environment Agency is delighted to be working in partnership with the Middleton Hatchery Group. These fish should thrive in the Lune and its tributaries, and are a major boost to the catchment.”